12339 1 ap  (Proteintech)

Journal: bioRxiv

Article Title: Parkinson’s disease risk factors are expressed at brain barriers

doi: 10.1101/2025.10.20.683561

Figure Lengend Snippet: A-B. Representative confocal images showing CAVIN2 (white), Collagen IV (green), TH (orange) expression in the SN (A) and frontal cortex (B) of human postmortem samples from controls and PD donors. C. Quantification of CAVIN2 signal intensity in collagen IV-positive blood vessels associated with acquisitions A and B. The violin plot shows the median (dashed line) and interquartile range (solid line). Data are from five patients and five control donors (n˃ 1,510 vessels analyzed for each condition). Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparisons correction. D. UMAPs represent CAVIN2 expression level in endothelial nuclei related to Smajić et al. and Kamath et al. E. Graph representing the proportion of CAVIN2 -expressing endothelial nuclei by snRNA-seq (blue bars), and CAVIN2-positive vascular fragments by immunofluorescence (purple bars). F-G. Representative confocal images showing the presence of CAVIN2 (red), Collagen IV (green), TH (white) in blood vessels of the mouse SN (F) , cortex and hippocampus (G ). White arrows point to CAVIN2-positive blood vessels. ( H ) Representative confocal images showing the presence of CAVIN2 (white) and COLLAGEN IV in mouse brain capillaries. Abbreviations: Ctl, control; PD, Parkinson’s disease.

Article Snippet: The following antibodies were used: rabbit anti-Annexin 1 (1:200, Invitrogen, 71-3400), rabbit anti-Annexin 1 (1:200, Abcam, ab214486), rabbit anti-SDPR (CAVIN2) (1:200, Bio-Techne, NBP2-57218), rabbit anti-SDPR (CAVIN2) (1:200, Proteintech, 12339-1-AP), rabbit anti-ANO2 (1:200, Bio-Techne, NBP2-92888), rabbit anti-ANO2 (1:200, Abcam, 113443), mouse anti-GFAP (1:500, Novus Biologicals, NBP1-05197).

Techniques: Expressing, Control, Immunofluorescence

Journal: bioRxiv

Article Title: Parkinson’s disease risk factors are expressed at brain barriers

doi: 10.1101/2025.10.20.683561

Figure Lengend Snippet: A-B. Representative confocal images showing CAVIN2 (white), Collagen IV (green), TH (orange) expression in the SN (A) and frontal cortex (B) of human postmortem samples from controls and PD donors. C. Quantification of CAVIN2 signal intensity in collagen IV-positive blood vessels associated with acquisitions A and B. The violin plot shows the median (dashed line) and interquartile range (solid line). Data are from five patients and five control donors (n˃ 1,510 vessels analyzed for each condition). Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparisons correction. D. UMAPs represent CAVIN2 expression level in endothelial nuclei related to Smajić et al. and Kamath et al. E. Graph representing the proportion of CAVIN2 -expressing endothelial nuclei by snRNA-seq (blue bars), and CAVIN2-positive vascular fragments by immunofluorescence (purple bars). F-G. Representative confocal images showing the presence of CAVIN2 (red), Collagen IV (green), TH (white) in blood vessels of the mouse SN (F) , cortex and hippocampus (G ). White arrows point to CAVIN2-positive blood vessels. ( H ) Representative confocal images showing the presence of CAVIN2 (white) and COLLAGEN IV in mouse brain capillaries. Abbreviations: Ctl, control; PD, Parkinson’s disease.

Article Snippet: The second CAVIN2 (Proteintech, 12339-1-AP) and ANXA1 (Invitrogen, 71-3400) antibodies provided similar staining patterns.

Techniques: Expressing, Control, Immunofluorescence